2e2i | pdb_00002e2i
RNA polymerase II elongation complex in 5 mM Mg+2 with 2'-dGTP
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| 2e2i, resolution 3.41Å | |||||||||||||
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| Ligands: | ZN, MG and DGT | ||||||||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Overview
New structures of RNA polymerase II (pol II) transcribing complexes reveal a likely key to transcription. The trigger loop swings beneath a correct nucleoside triphosphate (NTP) in the nucleotide addition site, closing off the active center and forming an extensive network of interactions with the NTP base, sugar, phosphates, and additional pol II residues. A histidine side chain in the trigger loop, precisely positioned by these interactions, may literally "trigger" phosphodiester bond formation. Recognition and catalysis are thus coupled, ensuring the fidelity of transcription.
About this Structure
2E2I is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structural basis of transcription: role of the trigger loop in substrate specificity and catalysis., Wang D, Bushnell DA, Westover KD, Kaplan CD, Kornberg RD, Cell. 2006 Dec 1;127(5):941-54. PMID:17129781
Page seeded by OCA on Sun Mar 23 14:58:49 2008
