2vb6 | pdb_00002vb6
MYOSIN VI (MD-INSERT2-CAM, DELTA INSERT1) POST-RIGOR STATE (CRYSTAL FORM 2)
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| 2vb6, resolution 2.30Å | |||||||||||||
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| Sites: | AC1, AC2, AC3, AC4, AC5, AC6 and AC7 | ||||||||||||
| Ligands: | MG, CA, ADP and BEF | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Overview
Myosin VI has an unexpectedly large swing of its lever arm (powerstroke) that optimizes its unique reverse direction movement. The basis for this is an unprecedented rearrangement of the subdomain to which the lever arm is attached, referred to as the converter. It is unclear at what point(s) in the myosin VI ATPase cycle rearrangements in the converter occur, and how this would effect lever arm position. We solved the structure of myosin VI with an ATP analogue (ADP.BeF3) bound in its nucleotide-binding pocket. The structure reveals that no rearrangement in the converter occur upon ATP binding. Based on previously solved myosin structures, our structure suggests that no reversal of the powerstroke occurs during detachment of myosin VI from actin. The structure also reveals novel features of the myosin VI motor that may be important in maintaining the converter conformation during detachment from actin, and other features that may promote rapid rearrangements in the structure following actin detachment that enable hydrolysis of ATP.
About this Structure
2VB6 is a Protein complex structure of sequences from Gallus gallus and Sus scrofa. Full crystallographic information is available from OCA.
Reference
The post-rigor structure of myosin VI and implications for the recovery stroke., Menetrey J, Llinas P, Cicolari J, Squires G, Liu X, Li A, Sweeney HL, Houdusse A, EMBO J. 2008 Jan 9;27(1):244-52. Epub 2007 Nov 29. PMID:18046460
Page seeded by OCA on Sun Mar 23 15:53:31 2008
