2iz9 | pdb_00002iz9

MS2-RNA HAIRPIN (4ONE-5) COMPLEX

spinqualitylabelsall models 2iz9, resolution 2.85Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2iz9 is a 5 chain structure with sequence from Bacteriophage ms2. This structure supersedes the now removed PDB entry 1dzs. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. NonStd Res: ONE Related: 1aq3, 1aq4, 1bms, 1msc, 1mst, 1mva, 1mvb, 1u1y, 1zdh, 1zdi, 1zdj, 1zdk, 1zse, 2b2d, 2b2e, 2b2g, 2bny, 2bq5, 2bs0, 2bs1, 2bu1, 2c4q, 2c4y, 2c4z, 2c50, 2c51, 2ms2, 5msf, 6msf, 7msf, 2iz8, 2izm, 2izn Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Function [COAT_BPMS2] Forms the phage shell; binds to the phage RNA. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The MS2 coat protein binds specifically to an RNA hairpin formed within the viral genome. By soaking different RNA fragments into crystals of MS2 coat protein capsids it is possible to determine the X-ray structure of the RNA-protein complexes formed. Here we present the structure to 2.85 A resolution of a complex between a chemically modified RNA hairpin variant and the MS2 coat protein. This RNA variant has a substitution at the -5 base position, which has been shown previously to be pyrimidine-specific and is a uracil in the wild-type RNA. The modified RNA hairpin contains a pyridin-4-one base (4one) at this position that lacks the exocyclic 2-oxygen eliminating the possibility of forming a hydrogen bond to asparagine A87 in the protein. The 4one complex structure shows an unprecedented major conformational change in the loop region of the RNA, whereas there is almost no change in the conformation of the protein. Deletion of a single hydrogen bonding atom from the MS2 RNA operator leads to dramatic rearrangements at the RNA-coat protein interface.,Grahn E, Stonehouse NJ, Adams CJ, Fridborg K, Beigelman L, Matulic-Adamic J, Warriner SL, Stockley PG, Liljas L Nucleic Acids Res. 2000 Dec 1;28(23):4611-6. PMID:11095669 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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