1k6d | pdb_00001k6d
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| 1k6d, resolution 1.90Å | |||||||||||||
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| Ligands: | MG | ||||||||||||
| Activity: | Acetate CoA-transferase, with EC number 2.8.3.8 | ||||||||||||
| Domains: | PRK09920 | ||||||||||||
| Resources: | FirstGlance, OCA, PDBsum, JenaLib, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT
Overview
The automation of protein structure determination is an essential component for high-throughput structural analysis in protein X-ray crystallography and is a key element in structural genomics. This highly challenging undertaking relies at present on the availability of high-quality native and derivatized protein crystals diffracting to high or moderate resolution, respectively. Obtaining such crystals often requires significant effort. The present study demonstrates that phases obtained at low resolution (>3.0 A) from crystals of SeMet-labeled protein can be successfully used for automated structure determination. The crystal structure of acetate CoA-transferase alpha-subunit was solved using 3.4 A multi-wavelength anomalous dispersion data collected from a crystal containing SeMet-substituted protein and 1.9 A data collected from a native protein crystal.
About this Structure
1K6D is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Autotracing of Escherichia coli acetate CoA-transferase alpha-subunit structure using 3.4 A MAD and 1.9 A native data., Korolev S, Koroleva O, Petterson K, Gu M, Collart F, Dementieva I, Joachimiak A, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2116-21. Epub 2002, Nov 23. PMID:12454473
Page seeded by OCA on Wed Mar 26 05:54:50 2008
