2quw | pdb_00002quw

Hammerhead Ribozyme G12A mutant after cleavage

spinqualitylabelsall models 2quw, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2quw is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MG NonStd Res: CCC, GTP Related: 2goz, 2qus Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Publication Abstract from PubMed We have obtained precatalytic (enzyme-substrate complex) and postcatalytic (enzyme-product complex) crystal structures of an active full-length hammerhead RNA that cleaves in the crystal. Using the natural satellite tobacco ringspot virus hammerhead RNA sequence, the self-cleavage reaction was modulated by substituting the general base of the ribozyme, G12, with A12, a purine variant with a much lower pKa that does not significantly perturb the ribozyme's atomic structure. The active, but slowly cleaving, ribozyme thus permitted isolation of enzyme-substrate and enzyme-product complexes without modifying the nucleophile or leaving group of the cleavage reaction, nor any other aspect of the substrate. The predissociation enzyme-product complex structure reveals RNA and metal ion interactions potentially relevant to transition-state stabilization that are absent in precatalytic structures. Capturing hammerhead ribozyme structures in action by modulating general base catalysis.,Chi YI, Martick M, Lares M, Kim R, Scott WG, Kim SH PLoS Biol. 2008 Sep 30;6(9):e234. PMID:18834200 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribozyme

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