4zon | pdb_00004zon

Structure of FtmOx1 with fumitremorgen B complex

spinqualitylabelsall models 4zon, resolution 2.11Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4zon is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 4Q1, CO, FE2 Related: 4y5t, 4y5s Activity: Verruculogen synthase, with EC number 1.14.11.38 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Function [FTMF_ASPFU] Catalyzes the conversion of fumitremorgin B to verruculogen. Publication Abstract from PubMed Many peroxy-containing secondary metabolites have been isolated and shown to provide beneficial effects to human health. Yet, the mechanisms of most endoperoxide biosyntheses are not well understood. Although endoperoxides have been suggested as key reaction intermediates in several cases, the only well-characterized endoperoxide biosynthetic enzyme is prostaglandin H synthase, a haem-containing enzyme. Fumitremorgin B endoperoxidase (FtmOx1) from Aspergillus fumigatus is the first reported alpha-ketoglutarate-dependent mononuclear non-haem iron enzyme that can catalyse an endoperoxide formation reaction. To elucidate the mechanistic details for this unique chemical transformation, we report the X-ray crystal structures of FtmOx1 and the binary complexes it forms with either the co-substrate (alpha-ketoglutarate) or the substrate (fumitremorgin B). Uniquely, after alpha-ketoglutarate has bound to the mononuclear iron centre in a bidentate fashion, the remaining open site for oxygen binding and activation is shielded from the substrate or the solvent by a tyrosine residue (Y224). Upon replacing Y224 with alanine or phenylalanine, the FtmOx1 catalysis diverts from endoperoxide formation to the more commonly observed hydroxylation. Subsequent characterizations by a combination of stopped-flow optical absorption spectroscopy and freeze-quench electron paramagnetic resonance spectroscopy support the presence of transient radical species in FtmOx1 catalysis. Our results help to unravel the novel mechanism for this endoperoxide formation reaction. Endoperoxide formation by an alpha-ketoglutarate-dependent mononuclear non-haem iron enzyme.,Yan W, Song H, Song F, Guo Y, Wu CH, Sae Her A, Pu Y, Wang S, Naowarojna N, Weitz A, Hendrich MP, Costello CE, Zhang L, Liu P, Jessie Zhang Y Nature. 2015 Nov 26;527(7579):539-43. doi: 10.1038/nature15519. Epub 2015 Nov 2. PMID:26524521 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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