4r4p | pdb_00004r4p

Crystal Structure of the VS ribozyme-A756G mutant

spinqualitylabelsall models 4r4p, resolution 3.07Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4r4p is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MG NonStd Res: GDP Related: 4r4v Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Publication Abstract from PubMed The Varkud satellite (VS) ribozyme mediates rolling-circle replication of a plasmid found in the Neurospora mitochondrion. We report crystal structures of this ribozyme from Neurospora intermedia at 3.1 A resolution, which revealed an intertwined dimer formed by an exchange of substrate helices. In each protomer, an arrangement of three-way helical junctions organizes seven helices into a global fold that creates a docking site for the substrate helix of the other protomer, resulting in the formation of two active sites in trans. This mode of RNA-RNA association resembles the process of domain swapping in proteins and has implications for RNA regulation and evolution. Within each active site, adenine and guanine nucleobases abut the scissile phosphate, poised to serve direct roles in catalysis. Similarities to the active sites of the hairpin and hammerhead ribozymes highlight the functional importance of active-site features, underscore the ability of RNA to access functional architectures from distant regions of sequence space, and suggest convergent evolution. Crystal structure of the Varkud satellite ribozyme.,Suslov NB, DasGupta S, Huang H, Fuller JR, Lilley DM, Rice PA, Piccirilli JA Nat Chem Biol. 2015 Nov;11(11):840-6. doi: 10.1038/nchembio.1929. Epub 2015 Sep, 28. PMID:26414446 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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