3jbb | pdb_00003jbb

Characterization of red-shifted phycobiliprotein complexes isolated from the chlorophyll f-containing cyanobacterium Halomicronema hongdechloris

spinqualitylabelsall models 3jbb, resolution 26.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3jbb is a 12 chain structure with sequence from Halomicronema hongdechloris. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CYC, SO4 NonStd Res: MEN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Publication Abstract from PubMed Phycobilisomes are the main light-harvesting protein complexes in cyanobacteria and some algae. It is commonly accepted that these complexes only absorb green and orange light, complementing chlorophyll absorbance. Here, we present a new phycobilisome derived complex that consists only of allophycocyanin core subunits, having red-shifted absorption peaks of 653 and 712nm. These red-shifted phycobiliprotein complexes were isolated from the chlorophyll f-containing cyanobacterium, Halomicronema hongdechloris, grown under monochromatic 730nm-wavelength (far-red) light. The 3D model obtained from single particle analysis reveals a double disk assembly of 120-145A with two alpha/beta allophycocyanin trimers fitting into the two separated disks. They are significantly smaller than typical phycobilisomes formed from allophycocyanin subunits and core-membrane linker proteins, which fit well with a reduced distance between thylakoid membranes observed from cells grown under far-red light. Spectral analysis of the dissociated and denatured phycobiliprotein complexes grown under both these light conditions shows that the same bilin chromophore, phycocyanobilin, is exclusively used. Our findings show that red-shifted phycobilisomes are required for assisting efficient far-red light harvesting. Their discovery provides new insights into the molecular mechanisms of light harvesting under extreme conditions for photosynthesis, as well as the strategies involved in flexible chromatic acclimation to diverse light conditions. Characterization of red-shifted phycobilisomes isolated from the chlorophyll f-containing cyanobacterium Halomicronema hongdechloris.,Li Y, Lin Y, Garvey CJ, Birch D, Corkery RW, Loughlin PC, Scheer H, Willows RD, Chen M Biochim Biophys Acta. 2015 Oct 26;1857(1):107-114. doi:, 10.1016/j.bbabio.2015.10.009. PMID:26514405 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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