2a2f | pdb_00002a2f

Crystal Structure of Sec15 C-terminal domain

spinqualitylabelsall models 2a2f, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2a2f is a 1 chain structure with sequence from Drome. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: sec15 (DROME) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Function [EXOC6_DROME] Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Sec15, a component of the exocyst, recognizes vesicle-associated Rab GTPases, helps target transport vesicles to the budding sites in yeast and is thought to recruit other exocyst proteins. Here we report the characterization of a 35-kDa fragment that comprises most of the C-terminal half of Drosophila melanogaster Sec15. This C-terminal domain was found to bind a subset of Rab GTPases, especially Rab11, in a GTP-dependent manner. We also provide evidence that in fly photoreceptors Sec15 colocalizes with Rab11 and that loss of Sec15 affects rhabdomere morphology. Determination of the 2.5-A crystal structure of the C-terminal domain revealed a novel fold consisting of ten alpha-helices equally distributed between two subdomains (N and C subdomains). We show that the C subdomain, mainly via a single helix, is sufficient for Rab binding. Sec15 interacts with Rab11 via a novel domain and affects Rab11 localization in vivo.,Wu S, Mehta SQ, Pichaud F, Bellen HJ, Quiocho FA Nat Struct Mol Biol. 2005 Oct;12(10):879-85. Epub 2005 Sep 11. PMID:16155582 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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