2w88 | pdb_00002w88

PLASTOCYANIN VARIANT WITH N-TERMINAL METHIONINE - OPEN STRUCTURE

spinqualitylabelsall models 2w88, resolution 2.89Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2w88 is a 3 chain structure with sequence from Phola. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CU, ZN Related: 1baw Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The beta-sandwich cupredoxin Plastocyanin (Pc) was found to self-assemble in the presence of Zn(2+), a known mediator of protein-protein interfaces. Diffraction-quality crystals of Pc grew from solutions containing zinc acetate as the sole precipitant. Di- and trinuclear zinc sites contribute to the crystal contacts in this structure. A different crystal form, also involving numerous zinc bridging ions, was obtained in the presence of poly(ethylene glycol) 8 000. Comparison of the two crystal forms reveals the effect of macromolecular crowding on self-assembly. Solution-state structural characterisation of the Zn(2+)-mediated Pc oligomers was performed by using a combination of chemical shift perturbation mapping and small-angle X-ray scattering. The data indicate the formation of dimers in solution. The implications for metal-mediated assembly and crystallisation are discussed. Metal-Mediated Self-Assembly of a beta-Sandwich Protein.,Crowley PB, Matias PM, Khan AR, Roessle M, Svergun DI Chemistry. 2009 Oct 15. PMID:19834935 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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