5eq3 | pdb_00005eq3

Crystal structure of the SrpA adhesin from Streptococcus sanguinis with a sialyl galactose disaccharide bound

spinqualitylabelsall models 5eq3, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5eq3 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ACT, CA, MBG, NA, NGC Related: 5eq2 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Publication Abstract from PubMed Streptococcus sanguinis is a leading cause of infective endocarditis, a life-threatening infection of the cardiovascular system. An important interaction in the pathogenesis of infective endocarditis is attachment of the organisms to host platelets. S. sanguinis expresses a serine-rich repeat adhesin, SrpA, similar in sequence to platelet-binding adhesins associated with increased virulence in this disease. In this study, we determined the first crystal structure of the putative binding region of SrpA (SrpABR) both unliganded and in complex with a synthetic disaccharide ligand at 1.8 A and 2.0 A resolution, respectively. We identified a conserved sequence Thr-Arg motif that orients the sialic acid moiety and is required for binding to platelet monolayers. Further, we propose that sequence insertions in closely related family members contribute to the modulation of structural and functional properties, including the quaternary structure, the tertiary structure, and the ligand binding site. Structural Basis for Sialoglycan Binding by the Streptococcus sanguinis SrpA Adhesin.,Bensing BA, Loukachevitch LV, McCulloch KM, Yu H, Vann KR, Wawrzak Z, Anderson S, Chen X, Sullam PM, Iverson TM J Biol Chem. 2016 Feb 1. pii: jbc.M115.701425. PMID:26833566 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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