Sandbox Reserved 428
| This Sandbox is Reserved from January 19, 2016, through August 31, 2016 for use for Proteopedia Team Projects by the class Chemistry 423 Biochemistry for Chemists taught by Lynmarie K Thompson at University of Massachusetts Amherst, USA. This reservation includes Sandbox Reserved 425 through Sandbox Reserved 439. |
Vitamin D receptor/vitamin D (1db1)[1]
by Roger Crocker, Kate Daborowski, Patrick Murphy, Benjamin Rizkin and Aaron Thole
Student Projects for UMass Chemistry 423 Spring 2016
<StructureSection load='1db1' size='350' side='right' caption='caption for Molecular Playground (PDB entry 1db1)' scene=>
Introduction
What to talk about:
What class of proteins it belongs to, how its characterized, other names
-Nuclear hormone receptor
-Component of Mediator complex
Basic function
-transcription regulator of hormone sensitive genes
-has central role in calcium homeostasis
Retailed Diseases
-Rickets, hypocalcemia, secondary hyperparathyroidism, total alopecia
Importance
Amino/Carboxy Color Scene
Overall Structure
Diagram of Protein Protein indicates alpha helices, while yellow indicates beta sheets: Outline: -Discuss Primary Structure[ -Discuss Secondary Structure Use this scene -Comprised of alpha helices and 1 beta sheet -Discuss tertiary structure -No quaternary structure -427 amino acids, 48289 Da (https://www.genecards.org/cgi-bin/carddisp.pl?gene=VDR)
The vitamin d receptor contains 427 amino acids with a total molecular weight of 48,289 Da [
Binding Interactions
Protein 1db1 is found to complex with 1,25 Dihydroxy Vitamin D3. This molecule has three notable alcohol groups that have the ability to participate in interactions with the protein. Vitamin D3 has a large number of relations with the residues on the protein chain. First in the sequence are residues 140-151. Tyr143, shown in blue, is the closest to the ligand at 2.83 angstroms. This is sightly large but there is still the possibility of hydrogen bonding. Tyr147 in green and Phe150 in black are also known to have interactions with Vitamin D3 they are farther away and therefore less significant. Outline: as has been started, the interactions of the various residues will be covered. The types of bonds that 1db1 and VDX form will be elaborated as well
Additional Features
A mutation in the transcription of the protein has the potential to result in the disease known as type 2 rickets. The mutation results in the Heterodimer not forming properly. Therefore VDR cannot complex with VDX. Outline: -Discuss mutations in Vitamin D receptor (Rickets) -Regulating hair cycle -Examine attempts to regulate Vitamin D receptor
Quiz Question 1
Quiz question: More information is needed to focus what the quiz should be about. Possibly related to how the secondary structure is comprised almost entirely of alpha helices, and how that relates to the amino acid sequences.
See Also
Credits
Introduction - Kate Daborowski
Overall Structure - Aaron Thole and Benjamin Rizkin
Drug Binding Site - Roger Crocker
Additional Features - Patrick Murphy
Quiz Question 1 - name of team member
