5cr4 | pdb_00005cr4

Crystal structure of the Sleeping Beauty transposase catalytic domain

spinqualitylabelsall models 5cr4, resolution 1.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5cr4 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CIT, EPE, GOL, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Publication Abstract from PubMed Sleeping Beauty (SB) is a prominent Tc1/mariner superfamily DNA transposon that provides a popular genome engineering tool in a broad range of organisms. It is mobilized by a transposase enzyme that catalyses DNA cleavage and integration at short specific sequences at the transposon ends. To facilitate SB's applications, here we determine the crystal structure of the transposase catalytic domain and use it to model the SB transposase/transposon end/target DNA complex. Together with biochemical and cell-based transposition assays, our structure reveals mechanistic insights into SB transposition and rationalizes previous hyperactive transposase mutations. Moreover, our data enables us to design two additional hyperactive transposase variants. Our work provides a useful resource and proof-of-concept for structure-based engineering of tailored SB transposases. Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering.,Voigt F, Wiedemann L, Zuliani C, Querques I, Sebe A, Mates L, Izsvak Z, Ivics Z, Barabas O Nat Commun. 2016 Mar 30;7:11126. doi: 10.1038/ncomms11126. PMID:27025571 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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