5i28 | pdb_00005i28

Azurin T30R1, crystal form II

spinqualitylabelsall models 5i28, resolution 1.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5i28 is a 16 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CU, GOL NonStd Res: R1A Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Function [AZUR_PSEAE] Transfers electrons from cytochrome c551 to cytochrome oxidase. Publication Abstract from PubMed Pulsed electron-electron double resonance (PELDOR or DEER) in combination with site-directed spin labelling has emerged as an important method for measuring nanometer distance constraints that are used to obtain coarse-grained structures of biomolecules or to follow their conformational changes. Translating measured spin-spin distances between spin labels into structural information requires taking the conformational flexibility of spin label side chains into account. Here, we present an analysis of orientation selective PELDOR data recorded on six singly MTSSL-labelled azurin mutants. The analysis yielded conformational MTSSL ensembles, which are considerably narrower than those predicted using in silico spin labeling methods but match well with spin label conformations found in the corresponding crystal structures. The possible reasons and consequences for predicting spin label conformers in the fold of biomolecules are discussed. Determination of nitroxide spin label conformations via PELDOR and X-ray crystallography.,Abdullin D, Hagelueken G, Schiemann O Phys Chem Chem Phys. 2016 Apr 21;18(15):10428-37. doi: 10.1039/c6cp01307d. Epub, 2016 Mar 31. PMID:27029516 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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