5f56 | pdb_00005f56

Structure of RecJ complexed with DNA and SSB-ct

spinqualitylabelsall models 5f56, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5f56 is a 3 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MN, SO4 Related: 5f54, 5f55 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Publication Abstract from PubMed The resection of DNA strand with a 5 end at double-strand breaks is an essential step in recombinational DNA repair. RecJ, a member of DHH family proteins, is the only 5 nuclease involved in the RecF recombination pathway. Here, we report the crystal structures of Deinococcus radiodurans RecJ in complex with deoxythymidine monophosphate (dTMP), ssDNA, the C-terminal region of single-stranded DNA-binding protein (SSB-Ct) and a mechanistic insight into the RecF pathway. A terminal 5 -phosphate-binding pocket above the active site determines the 5 -3 polarity of the deoxy-exonuclease of RecJ; a helical gateway at the entrance to the active site admits ssDNA only; and the continuous stacking interactions between protein and nine nucleotides ensure the processive end resection. The active site of RecJ in the N-terminal domain contains two divalent cations that coordinate the nucleophilic water. The ssDNA makes a 180 degrees turn at the scissile phosphate. The C-terminal domain of RecJ binds the SSB-Ct, which explains how RecJ and SSB work together to efficiently process broken DNA ends for homologous recombination. Structural basis for DNA 5 -end resection by RecJ.,Cheng K, Xu H, Chen X, Wang L, Tian B, Zhao Y, Hua Y Elife. 2016 Apr 8;5. pii: e14294. doi: 10.7554/eLife.14294. PMID:27058167 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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