5l8z | pdb_00005l8z

Structure of thermostable DNA-binding HU protein from micoplasma Spiroplasma melliferum

spinqualitylabelsall models 5l8z, resolution 1.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5l8z is a 1 chain structure. This structure supersedes the now removed PDB entry 5cvx. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Publication Abstract from PubMed HU proteins belong to the nucleoid-associated proteins (NAPs) that are involved in vital processes such as DNA compaction and reparation, gene transcription etc. No data are available on the structures of HU proteins from mycoplasmas. To this end, the HU protein from the parasitic mycoplasma Spiroplasma melliferum KC3 was cloned, overexpressed in Escherichia coli and purified to homogeneity. Prismatic crystals of the protein were obtained by the vapour-diffusion technique at 4 degrees C. The crystals diffracted to 1.36 A resolution (the best resolution ever obtained for a HU protein). The diffraction data were indexed in space group C2 and the structure of the protein was solved by the molecular-replacement method with one monomer per asymmetric unit. Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the histone-like HU protein from Spiroplasma melliferum KC3.,Boyko K, Gorbacheva M, Rakitina T, Korzhenevskiy D, Vanyushkina A, Kamashev D, Lipkin A, Popov V Acta Crystallogr F Struct Biol Commun. 2015 Jan 1;71(Pt 1):24-7. doi:, 10.1107/S2053230X14025333. Epub 2015 Jan 1. PMID:25615963 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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