Resolvase

Function

Resolvase or recombinase (Rec) is a nuclease which is involved in DNA recombination. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase.

• Holliday junction resolvase (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands.
  
• Tyr-recombinases include Cre which recombines loxP sites, FLP and lambda integrase (LamInt).
  
• Ser-recombinases (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase.
  
• RadA recombinase promotes DNA recombination. Detailed analysis of its dimeric structure suggests mechanisms for junction isomerization and communication between the two active sites .
  
• Hin-recombinase (HRec) is a protein of Salmonella. HRec inverts a 900 base pair DNA segment which contains the promoters of flagellar genes. The inversion changes the these genes' expressions.

3D structures of resolvase

Updated on 04-August-2016

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• Holliday junction resolvase
  • 2wiw, 2wiz, 2wj0 – AfHJR+DNA – Archaeoglobus fulgidus
    
  • 2wcw, 2wcz – AfHJR (mutant)
    
  • 2h8c – EcHJR RusA (mutant) + DNA – Escherichia coli
    
  • 1gdt - EcHJR +DNA
    
  • 2h8e - EcHJR RusA (mutant)
    
  • 1q8r – EcHJR RusA
    
  • 1hjr – EcHJR RuvC
    
  • 4ep4 – TtHJR RuvC – Thermus thermophilus
    
  • 4ep5 – TtHJR RuvC (mutant)
    
  • 1zp7 – HJR RecU – Bacillus subtilis
    
  • 1ob8, 1ob9, 1hh1 – SsHJR – Sulfolobus solfataricus
    
  • 2fco – HJR – Geobacillus kaustophilus
    
  • 1ipi, 1gef – HJR – Pyrococcus furiosus
    
  • 1kcf – HJR – fission yeast
    
• Ser-recombinase
  • 2rsl - EcGDR
    
  • 1hx7 – EcGDR N-terminal – NMR
    
  • 1ght, 1res, 1ret, 1ght – EcGDR catalytic domain – NMR
    
  • 1gdr, 1hx7 - EcGDR catalytic domain
    
  • 2gm4, 1zr2, 1zr4, 1gdt – EcGDR+DNA
    
  • 2gm5 – EcGDR (mutant)
    
  • 3guv – site-specific recombinase – Streptococcus pneumoniae
    
  • 2r0q – S-rec+DNA – Staphylococcus aureus
    
  • 3lhf – SsS-rec
    
• Tyr-recombinase
  • 3etl, 3ew9, 3ewa, 2i1q – MmRadA Rec+AMPPNP+ion – Methanococcus maripaludis
    
  • 2b21 - MmRadA Rec+AMPPNP
    
  • 2zub, 2zuc, 2zud, 2dfl – SsRadA
    
  • 2cvf, 2cvh – RadB Rec – Thermococcus kodakarensis
    
  • 2f1h, 2fpl, 2fpm - MvRec+AMPPNP+K – Methanococcus voltae
    
  • 2f1i – MvRec+AMPPNP
    
  • 2f1j, 2fpk - MvRec+ADP
    
  • 1a0p – EcXerd Rec
    
• Cre recombinase
  • 3mgv, 3c28, 3c29, 2hof, 2hoi, 1xns, 1xo0, 1pvp, 1pvq, 1pvr, 1nzb, 1ouq, 1q3u, 1q3v, 1ma7, 1kbu, 1drg, 1f44, 2crx, 3crx, 4crx, 5crx, 1crx – EpCre+DNA – Enterobacteria phage PI
    
• Hin recombinase
  • 1hcr, 1ijw, 1ijj6, 1jj8, 1jko, 1jkp, 1jkq, 1jkr – HRec + DNA – synthetic
    
• Integrase
  • 2a3v – Rec INTI4+DNA – Vibrio cholerae
    
  • 3bvp – Integrase N-terminal – Lactococcus phage
    
  • 2oxo – LamInt binding domain – phage
    
  • 1z19 – EpLamInt core binding +catalytic domain+DNA
    
  • 1z1b, 1z1g, 1p7d – EpLamInt+DNA
    
  • 1kjk – EpLamInt N-terminal – NMR
    
  • 1ae9 – EpLamInt catalytic core
    
  • 1p4e, 1m6x – yFLP (mutant)+DNA – yeast
    
  • 1flo - yFLP +DNA
    
  • 1aih – HP1 integrase catalytic domain - bacteriophage
    
  • 3nrw – INT/site-specific recombinase N-terminal – Haloarcula marismortui
    
  • 3jtz – INT arm-type binding domain – Yersinia pestis
    
  • 3ju0 – HPI INT arm-type binding domain – Pectobacterium atrosepticum
    
  • 2kkp – INT SAM-like domain – Moorella thermoacetica – NMR
    
  • 2kkv – INT fragment – Salmonella enterica – NMR
    
  • 2khq - INT fragment – Staphylococcus saprophyticus – NMR
    
  • 2wcc – λINT DBD+ DNA – Enterobacteria phage λ - NMR
    
  • 2khv, 2kj5 – INT residues 102-199 – Nitrosospira multiformis – NMR
    
• Recombinase A (RecA) see Recombinase A

Proteopedia Page Contributors and Editors (what is this?)

Alexander Berchansky, Jaime Prilusky, Joel L. Sussman, Michal Harel