3nzm | pdb_00003nzm

Crystal structure of DNAE intein with N-extein in redox trap

spinqualitylabelsall models 3nzm, resolution 1.55Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3nzm is a 1 chain structure with sequence from Aphanocapsa sp. (strain n-1). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: SO4 NonStd Res: SNN Related: 1zde, 1zd7 Gene: dnaE-N, slr0603, dnaE-C, sll1572 (Aphanocapsa sp. (strain N-1)) Activity: DNA-directed DNA polymerase, with EC number 2.7.7.7 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Here we describe self-splicing proteins, called inteins, that function as redox-responsive switches in bacteria. Redox regulation was achieved by engineering a disulfide bond between the intein's catalytic cysteine and a cysteine in the flanking 'extein' sequence. This interaction was validated by an X-ray structure, which includes a transient splice junction. A natural analog of the designed system was identified in Pyrococcus abyssi, suggesting an unprecedented form of adaptive, post-translational regulation. Structure of catalytically competent intein caught in a redox trap with functional and evolutionary implications.,Callahan BP, Topilina NI, Stanger MJ, Van Roey P, Belfort M Nat Struct Mol Biol. 2011 May;18(5):630-3. Epub 2011 Apr 3. PMID:21460844 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also DNA polymerase

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