3sxb | pdb_00003sxb

Crystal structure of ABBB+UDP+Gal with MPD as the cryoprotectant

spinqualitylabelsall models 3sxb, resolution 1.49Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3sxb is a 1 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GAL, MN, PEG, UDP Related: 3sx3, 3sx5, 3sx7, 3sx8, 3sxa, 3sxc, 3sxd, 3sxe, 3sxg Gene: ABO (HUMAN) Activity: Fucosylgalactoside 3-alpha-galactosyltransferase, with EC number 2.4.1.37 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [BGAT_HUMAN] This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. Publication Abstract from PubMed The human ABO(H) A and B blood group glycosyltransferases GTA and GTB differ by only four amino acids, yet this small dissimilarity is responsible for significant differences in biosynthesis, kinetics and structure. Like other glycosyltransferases, these two enzymes have been shown to recognize substrates through dramatic conformational changes in mobile polypeptide loops surrounding the active site. Structures of GTA, GTB and several chimeras determined by single-crystal X-ray diffraction demonstrate a range of susceptibility to the choice of cryoprotectant, in which the mobile polypeptide loops can be induced by glycerol to form the ordered closed conformation associated with substrate recognition and by MPD [hexylene glycol, (+/-)-2-methyl-2,4-pentanediol] to hinder binding of substrate in the active site owing to chelation of the Mn(2+) cofactor and thereby adopt the disordered open state. Glycerol is often avoided as a cryoprotectant when determining the structures of carbohydrate-active enzymes as it may act as a competitive inhibitor for monosaccharide ligands. Here, it is shown that the use of glycerol as a cryoprotectant can additionally induce significant changes in secondary structure, a phenomenon that could apply to any class of protein. Sequence-dependent effects of cryoprotectants on the active sites of the human ABO(H) blood group A and B glycosyltransferases.,Johal AR, Schuman B, Alfaro JA, Borisova S, Seto NO, Evans SV Acta Crystallogr D Biol Crystallogr. 2012 Mar;68(Pt 3):268-76. Epub 2012 Feb 14. PMID:22349229 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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