4gmp | pdb_00004gmp
Crystal structure of enterovirus 71 strain 1095 procapsid
Structural highlights
Function[E5RPG0_9ENTO] Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS001676_004_016611] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS001676_004_010047] Publication Abstract from PubMedEnterovirus 71 (EV71) is an important emerging human pathogen with a global distribution and presents a disease pattern resembling poliomyelitis with seasonal epidemics that include cases of severe neurological complications such as acute flaccid paralysis. EV71 is a member of the Picornaviridae family, which consists of icosahedral, non-enveloped, single-stranded RNA viruses. Here we report structures derived from X-ray crystallography and cryo-electron microscopy (cryo-EM) for the 1095 strain of EV71, including a putative precursor in virus assembly, the procapsid, and the mature virus capsid. The cryo-EM map of the procapsid provides new structural information on portions of capsid proteins VP0 and VP1 that are disordered in the higher resolution crystal structures. Our structures solved from virus particles in solution are largely in agreement with those from prior X-ray crystallographic studies, however, we observe small but significant structural differences for the 1095 procapsid compared to a structure solved by Wang et al, 2012, for a different strain of EV71. For both EV71 strains, the procapsid is significantly larger in diameter than mature capsid, unlike any other picornavirus. Nonetheless, our results demonstrate that picornavirus capsid expansion is possible without RNA encapsidation and that picornavirus assembly may involve an inward radial collapse of the procapsid to yield the native virion. The EV71 strain 1095 structures of procapsid and mature virion.,Cifuente JO, Lee H, Yoder JD, Shingler KL, Carnegie MS, Yoder JL, Ashley RE, Makhov AM, Conway JF, Hafenstein S J Virol. 2013 May 1. PMID:23637404[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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