3w0l | pdb_00003w0l

The crystal structure of Xenopus Glucokinase and Glucokinase Regulatory Protein complex

spinqualitylabelsall models 3w0l, resolution 2.92Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3w0l is a 4 chain structure with sequence from African clawed frog. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: F6R, NA, SO4 Gene: gck (African clawed frog), gckr (African clawed frog) Activity: Glucokinase, with EC number 2.7.1.2 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [GCKR_XENLA] Inhibits glucokinase by forming an inactive complex with this enzyme. Publication Abstract from PubMed Glucokinase (GK) is a monomeric allosteric enzyme and plays a pivotal role in blood glucose homeostasis. GK is regulated by GK regulatory protein (GKRP), and indirectly by allosteric effectors of GKRP. Despite the critical roles of GK and GKRP, the molecular basis for the allosteric regulation mechanism of GK by GKRP remains unclear. We determined the crystal structure of Xenopus GK and GKRP complex in the presence of fructose-6-phosphate at 2.9 A. GKRP binds to a super-open conformation of GK mainly through hydrophobic interaction, inhibiting the GK activity by locking a small domain of GK. We demonstrate the molecular mechanism for the modulation of GK activity by allosteric effectors of GKRP. Importantly, GKRP releases GK in a sigmoidal manner in response to glucose concentration by restricting a structural rearrangement of the GK small domain via a single ion pair. We find that GKRP acts as an allosteric switch for GK in blood glucose control by the liver. Molecular basis for the role of glucokinase regulatory protein as the allosteric switch for glucokinase.,Choi JM, Seo MH, Kyeong HH, Kim E, Kim HS Proc Natl Acad Sci U S A. 2013 Jun 18;110(25):10171-6. doi:, 10.1073/pnas.1300457110. Epub 2013 Jun 3. PMID:23733961 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Glucokinase Regulatory Protein Hexokinase

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