4oqe | pdb_00004oqe

Crystal structure of the tylM1 N,N-dimethyltransferase in complex with SAH and TDP-Fuc3NMe

spinqualitylabelsall models 4oqe, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4oqe is a 2 chain structure with sequence from "actinomyces_fradii"_(sic)_waksman_and_curtis_1916 "actinomyces fradii" (sic) waksman and curtis 1916. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MMF, SAH Related: 4oqd Gene: tylM1, tylMI ("Actinomyces fradii" (sic) Waksman and Curtis 1916) Activity: dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase, with EC number 2.1.1.235 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [TYLM1_STRFR] S-adenosyl-L-methionine-dependent methyltransferase involved in the biosynthesis of mycaminose, an essential structural component of the macrolide antibiotic tylosin. Involved in the last step in mycaminose biosynthesis by mediating dimethylation of the hexose C-3' amino group. Publication Abstract from PubMed The importance of unusual deoxysugars in biology has become increasingly apparent over the past decade. Some, for example, play key roles in the physiological activities of the natural products to which they are attached. Here we describe a study of TylM1, a dimethyltransferase from Streptomyces fradiae involved in the production of dTDP-mycaminose. From this investigation, the manner in which the enzyme binds its dimethylated product has been revealed. More significantly, by providing the enzyme with an alternative substrate, it was possible to produce a monomethylated product not observed in nature. This has important ramifications for the production of unique carbohydrates that may prove useful in drug design. Production of a novel N-monomethylated dideoxysugar.,Thoden JB, Holden HM Biochemistry. 2014 Feb 25;53(7):1105-7. doi: 10.1021/bi500098a. Epub 2014 Feb 11. PMID:24512254 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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