4lnu | pdb_00004lnu

Nucleotide-free kinesin motor domain in complex with tubulin and a DARPin

spinqualitylabelsall models 4lnu, resolution 2.19Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4lnu is a 4 chain structure with sequence from [1] and Ovis aries. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GDP, GOL, GTP, MES, MG, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [KINH_HUMAN] Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes (By similarity). Publication Abstract from PubMed Kinesin-1 is a dimeric ATP-dependent motor protein that moves towards microtubules (+) ends. This movement is driven by two conformations (docked and undocked) of the two motor domains carboxy-terminal peptides (named neck linkers), in correlation with the nucleotide bound to each motor domain. Despite extensive data on kinesin-1, the structural connection between its nucleotide cycle and movement has remained elusive, mostly because the structure of the critical tubulin-bound apo-kinesin state was unknown. Here we report the 2.2 A structure of this complex. From its comparison with detached kinesin-ADP and tubulin-bound kinesin-ATP, we identify three kinesin motor subdomains that move rigidly along the nucleotide cycle. Our data reveal how these subdomains reorient on binding to tubulin and when ATP binds, leading respectively to ADP release and to neck linker docking. These results establish a framework for understanding the transformation of chemical energy into mechanical work by (+) end-directed kinesins. The structure of apo-kinesin bound to tubulin links the nucleotide cycle to movement.,Cao L, Wang W, Jiang Q, Wang C, Knossow M, Gigant B Nat Commun. 2014 Nov 14;5:5364. doi: 10.1038/ncomms6364. PMID:25395082 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Tubulin

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