3f52 | pdb_00003f52

Crystal structure of the clp gene regulator ClgR from C. glutamicum

spinqualitylabelsall models 3f52, resolution 1.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3f52 is a 2 chain structure with sequence from "micrococcus_glutamicus"_kinoshita_et_al._1958 "micrococcus glutamicus" kinoshita et al. 1958. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GOL Related: 3f51 Gene: cg2152, Cgl1962 ("Micrococcus glutamicus" Kinoshita et al. 1958) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Human pathogens of the genera Corynebacterium and Mycobacterium possess the transcriptional activator ClgR (clp gene regulator) which in Corynebacterium glutamicum has been shown to regulate the expression of the ClpCP protease genes. ClgR specifically binds to pseudo-palindromic operator regions upstream of clpC and clpP1P2. Here, we present the first crystal structure of a ClgR protein from C. glutamicum. The structure was determined from two different crystal forms to resolutions of 1.75 and 2.05 A, respectively. ClgR folds into a five-helix bundle with a helix-turn-helix motif typical for DNA-binding proteins. Upon dimerization the two DNA-recognition helices are arranged opposite to each other at the protein surface in a distance of approximately 30 A, which suggests that they bind into two adjacent major grooves of B-DNA in an anti-parallel manner. A binding pocket is situated at a strategic position in the dimer interface and could possess a regulatory role altering the positions of the DNA-binding helices. Crystal structure of the caseinolytic protease gene regulator, a transcriptional activator in actinomycetes.,Russo S, Schweitzer JE, Polen T, Bott M, Pohl E J Biol Chem. 2009 Feb 20;284(8):5208-16. Epub 2008 Nov 19. PMID:19019826 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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