4g91 | pdb_00004g91

CCAAT-binding complex from Aspergillus nidulans

spinqualitylabelsall models 4g91, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4g91 is a 3 chain structure with sequence from A. nidulans and Aspergillus nidulans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 1n1j, 4g92 Gene: hapB (A. nidulans), AN4034.2, ANIA_04034 (Aspergillus nidulans), AN6492.2 (Aspergillus nidulans) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The CCAAT box is a frequent element of eukaryotic promoters, and its specific recognition by the conserved heterotrimeric CCAAT-binding complex (CBC) constitutes a key step in promoter organization and regulation of transcription. Here, we report the crystal structures of the CBC from Aspergillus nidulans in the absence and in complex with double-stranded DNA at 1.8 A resolution. The histone-like subunits HapC and HapE induce nucleosome-like DNA bending by interacting with the sugar-phosphate backbone. Minor groove sensing and widening by subunit HapB tightly anchor the CBC to the CCAAT box, as shown by structural and biochemical data. Furthermore, crucial interactions of the DNA duplex with subunit HapB provide an explanation for the sequence specificity of the CBC. The herein-described mode of transcription factor binding answers the question of how histone proteins gained sequence specificity for the CCAAT box. DNA minor groove sensing and widening by the CCAAT-binding complex.,Huber EM, Scharf DH, Hortschansky P, Groll M, Brakhage AA Structure. 2012 Oct 10;20(10):1757-68. doi: 10.1016/j.str.2012.07.012. Epub 2012 , Aug 16. PMID:22902862 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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