3s8q | pdb_00003s8q

Crystal structure of the R-M controller protein C.Esp1396I OL operator complex

spinqualitylabelsall models 3s8q, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3s8q is a 4 chain structure with sequence from Enterobacter sp. rfl1396. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 3clc, 3g5g, 3fya Gene: esp1396IC (Enterobacter sp. RFL1396) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The controller protein C.Esp1396I regulates the timing of gene expression of the restriction-modification (RM) genes of the RM system Esp1396I. The molecular recognition of promoter sequences by such transcriptional regulators is poorly understood, in part because the DNA sequence motifs do not conform to a well-defined symmetry. We report here the crystal structure of the controller protein bound to a DNA operator site. The structure reveals how two different symmetries within the operator are simultaneously recognized by the homo-dimeric protein, underpinned by a conformational change in one of the protein subunits. The recognition of two different DNA symmetries through movement of a flexible loop in one of the protein subunits may represent a general mechanism for the recognition of pseudo-symmetric DNA sequences. Recognition of dual symmetry by the controller protein C.Esp1396I based on the structure of the transcriptional activation complex.,McGeehan JE, Ball NJ, Streeter SD, Thresh SJ, Kneale GG Nucleic Acids Res. 2011 Dec 30. PMID:22210861 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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