4fe9 | pdb_00004fe9

Crystal Structure of SusF from Bacteroides thetaiotaomicron

spinqualitylabelsall models 4fe9, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4fe9 is a 1 chain structure with sequence from Bactn. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: BGC, EDO, GLC, GOL, MTT, NA, PO4 Related: 4fch, 4fem Gene: BT_3699, susF (BACTN) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [SUSF_BACTN] Starch-binding protein present at the surface of the cell. Mediates starch-binding before starch transport in the periplasm for degradation. SusE and SusF do not constitute the major starch-binding proteins in starch degradation pathway. Has lower affinity for starch compared to SusE. Publication Abstract from PubMed Human colonic bacteria are necessary for the digestion of many dietary polysaccharides. The intestinal symbiont Bacteroides thetaiotaomicron uses five outer membrane proteins to bind and degrade starch. Here, we report the x-ray crystallographic structures of SusE and SusF, two outer membrane proteins composed of tandem starch specific carbohydrate-binding modules (CBMs) with no enzymatic activity. Examination of the two CBMs in SusE and three CBMs in SusF reveals subtle differences in the way each binds starch and is reflected in their Kds for both high molecular weight starch and small maltooligosaccharides. Thus, each site seems to have a unique starch preference that may enable these proteins to interact with different regions of starch or its breakdown products. Proteins similar to SusE and SusF are encoded in many other polysaccharide utilization loci that are possessed by human gut bacteria in the phylum Bacteroidetes. Thus, these proteins are likely to play an important role in carbohydrate metabolism in these abundant symbiotic species. Understanding structural changes that diversify and adapt related proteins in the human gut microbial community will be critical to understanding the detailed mechanistic roles that they perform in the complex digestive ecosystem. Multi-domain carbohydrate-binding proteins involved in Bacteroides thetaiotaomicron starch metabolism.,Cameron EA, Maynard MA, Smith CJ, Smith TJ, Koropatkin NM, Martens EC J Biol Chem. 2012 Aug 21. PMID:22910908 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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