3r0r | pdb_00003r0r

The 2.3 A structure of porcine circovirus 2

spinqualitylabelsall models 3r0r, resolution 2.35Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3r0r is a 1 chain structure with sequence from Pcv2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: SO4 Gene: cap, Cap, ORF2, cp (PCV2) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Porcine circovirus 2 (PCV2) is a T=1 non-enveloped icosahedral virus that has had severe impact on the swine industry. Here we report the crystal structure of an N-terminally truncated PCV2 virus-like particle at 2.3 A resolution, and the cryo-EM image reconstruction of a full length PCV2 virus-like particle at 9.6 A resolution. This is the first atomic structure of a Circovirus. The crystal structure revealed that the capsid protein fold is a canonical viral jelly-roll. The loops connecting the strands of the jelly-roll define the limited features of the surface. Sulfate ions interacting with the surface, and electrostatic potential calculations strongly suggest a heparan sulfate binding site that allows PCV2 to gain entry into the cell. The crystal structure also allowed previously determined epitopes of the capsid to be visualized. The cryo-EM image reconstruction showed that the location of the N-terminus, absent in the crystal structure, is inside the capsid. As the N-terminus was previously shown to be antigenic it may externalize through viral 'breathing'. The 2.3 A Structure of Porcine Circovirus 2.,Khayat R, Brunn N, Speir JA, Hardham JM, Ankenbauer RG, Schneemann A, Johnson JE J Virol. 2011 Jun 1. PMID:21632760 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Virus coat protein

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