4epb | pdb_00004epb

Final Urease Structure for Radiation Damage Experiment at 100 K

spinqualitylabelsall models 4epb, resolution 1.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4epb is a 3 chain structure with sequence from "aerobacter_aerogenes"_hormaeche_and_edwards_1958 "aerobacter aerogenes" hormaeche and edwards 1958. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NI NonStd Res: KCX Related: 4ep8, 4epd, 4epe Gene: ureC ("Aerobacter aerogenes" Hormaeche and Edwards 1958), ureB ("Aerobacter aerogenes" Hormaeche and Edwards 1958), ureA ("Aerobacter aerogenes" Hormaeche and Edwards 1958) Activity: Urease, with EC number 3.5.1.5 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The spatial distribution of radiation damage (assayed by increases in atomic B factors) to thaumatin and urease crystals at temperatures ranging from 25 to 300 K is reported. The nature of the damage changes dramatically at approximately 180 K. Above this temperature the role of solvent diffusion is apparent in thaumatin crystals, as solvent-exposed turns and loops are especially sensitive. In urease, a flap covering the active site is the most sensitive part of the molecule and nearby loops show enhanced sensitivity. Below 180 K sensitivity is correlated with poor local packing, especially in thaumatin. At all temperatures, the component of the damage that is spatially uniform within the unit cell accounts for more than half of the total increase in the atomic B factors and correlates with changes in mosaicity. This component may arise from lattice-level, rather than local, disorder. The effects of primary structure on radiation sensitivity are small compared with those of tertiary structure, local packing, solvent accessibility and crystal contacts. Spatial distribution of radiation damage to crystalline proteins at 25-300 K.,Warkentin M, Badeau R, Hopkins JB, Thorne RE Acta Crystallogr D Biol Crystallogr. 2012 Sep;68(Pt 9):1108-17. doi:, 10.1107/S0907444912021361. Epub 2012 Aug 18. PMID:22948911 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Urease

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