3ufd | pdb_00003ufd

C.Esp1396I bound to its highest affinity operator site OM

spinqualitylabelsall models 3ufd, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3ufd is a 8 chain structure with sequence from Enterobacter sp. rfl1396. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CL Related: 3clc, 3g5g, 3fya, 3s8q Gene: esp1396IC (Enterobacter sp. RFL1396) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Controller (C) proteins regulate the expression of restriction-modification (RM) genes in a wide variety of RM systems. However, the RM system Esp1396I is of particular interest as the C protein regulates both the restriction endonuclease (R) gene and the methyltransferase (M) gene. The mechanism of this finely tuned genetic switch depends on differential binding affinities for the promoters controlling the R and M genes, which in turn depends on differential DNA sequence recognition and the ability to recognize dual symmetries. We report here the crystal structure of the C protein bound to the M promoter, and compare the binding affinities for each operator sequence by surface plasmon resonance. Comparison of the structure of the transcriptional repression complex at the M promoter with that of the transcriptional activation complex at the R promoter shows how subtle changes in protein-DNA interactions, underpinned by small conformational changes in the protein, can explain the molecular basis of differential regulation of gene expression. The structural basis of differential DNA sequence recognition by restriction-modification controller proteins.,Ball NJ, McGeehan JE, Streeter SD, Thresh SJ, Kneale GG Nucleic Acids Res. 2012 Aug 31. PMID:22941636 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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