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Function
RmlC is an epimerase enzyme of the dTPD-rhamnose pathway. Deoxy-thymidine diphosphate (dTDP)-L-rhamnose is the precursor of L-rhamnose. L-rhamnose is a sugar component of microbial cell wall and is absent in human[1].
Disease
Relevance
Inhibitors of RmlC can serve as potential antimicrobial drugs[2].
Structural highlights
The active site of RmlC contains the reaction product DTDP-L-rhmnose[3].
- ↑ Giraud MF, Leonard GA, Field RA, Berlind C, Naismith JH. RmlC, the third enzyme of dTDP-L-rhamnose pathway, is a new class of epimerase. Nat Struct Biol. 2000 May;7(5):398-402. PMID:10802738 doi:10.1038/75178
- ↑ Kantardjieff KA, Kim CY, Naranjo C, Waldo GS, Lekin T, Segelke BW, Zemla A, Park MS, Terwilliger TC, Rupp B. Mycobacterium tuberculosis RmlC epimerase (Rv3465): a promising drug-target structure in the rhamnose pathway. Acta Crystallogr D Biol Crystallogr. 2004 May;60(Pt 5):895-902. Epub 2004, Apr 21. PMID:15103135 doi:10.1107/S0907444904005323
- ↑ Dong C, Major LL, Srikannathasan V, Errey JC, Giraud MF, Lam JS, Graninger M, Messner P, McNeil MR, Field RA, Whitfield C, Naismith JH. RmlC, a C3' and C5' carbohydrate epimerase, appears to operate via an intermediate with an unusual twist boat conformation. J Mol Biol. 2007 Jan 5;365(1):146-59. Epub 2006 Sep 29. PMID:17046787 doi:https://dx.doi.org/10.1016/j.jmb.2006.09.063
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3D structures of RmlC
Updated on 17-August-2016
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- RmlC
- 1pm7, 1upi – MtRmlC – Mycobacterium tuberculosis
- 2ixj, 1rtv – PaRmlC – Pseudomonas aeruginosa
- 1nxm – SsRmlC – Streptococcus suis
- 1ep0 – MetRmlC – Methanothermobacter thermautotrophicus
- 1dzr – StRmlC – Salmonella typhimurium
- RmlC complexes
- 2ixc – MtRmlC + dTDP rhamnose
- 2ixh – PaRmlC + dTDP rhamnose
- 2ixl – SsRmlC + dTDP rhamnose
- 2ixk – PaRmlC + dTDP-keto rhamnose
- 1nyw – SsRmlC + dTDP glucose
- 1nzc – SsRmlC + dTDP xylose
- 1epz – MetRmlC + TDP
- 3ryk – RmlC + TDP + pyrophosphate – Bacillus anthracis
- 1dzt – StRmlC + acetylthymidine diphosphate
References
