| Function
Tryptophan synthase (TrpS) is an α2β2 tetramer participating in the biosynthesis of tryptophan[1]. TrpS α subunit catalyzes the formation of indole and glyceraldehyde-3-phosphate (G3P) from indole-3-glycerol phosphate (IGP). The β subunit catalyzes in a pyridoxal-phosphate (PLP) dependent reaction the formation of tryptophan (Trp) from indole and serine. The diffusion of indole from the α to the β subunit is facilitated via a hydrophobic channel connecting the subunits.
Relevance
TrpS is not found in animals hence it is tested as a possible drug target for tuberculosis, ocular and genital infections, cryptosporidiosis and as herbicide[2].
Structural highlights
The active site of TrpS subunit α contains the catalytic residues Glu and Asp which interact with G3P[3].
- ↑ Miles EW. Tryptophan synthase: a multienzyme complex with an intramolecular tunnel. Chem Rec. 2001;1(2):140-51. PMID:11893063
- ↑ Shen H, Yang Y, Wang F, Zhang Y, Ye N, Xu S, Wang H. Characterization of the putative tryptophan synthase beta-subunit from Mycobacterium tuberculosis. Acta Biochim Biophys Sin (Shanghai). 2009 May;41(5):379-88. PMID:19430702
- ↑ Kulik V, Hartmann E, Weyand M, Frey M, Gierl A, Niks D, Dunn MF, Schlichting I. On the structural basis of the catalytic mechanism and the regulation of the alpha subunit of tryptophan synthase from Salmonella typhimurium and BX1 from maize, two evolutionarily related enzymes. J Mol Biol. 2005 Sep 23;352(3):608-20. PMID:16120446 doi:10.1016/j.jmb.2005.07.014
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3D structures of tryptophan synthase
Updated on 27-September-2016
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- Tryptophan synthase α subunit
- 3vnd - TrpS α subunit – Shewanella frigidimarina
- 3tha - TrpS α subunit – Campylobacter jejuni
- 3nav - TrpS α subunit – Vibrio cholerae
- 1geq - PfTrpS α subunit - Pyrococcus furiosus
- 2dzp, 2dzs, 2dzt, 2dzu, 2dzv, 2dzw, 2dzx, 2e09 - PfTrpS α subunit (mutant)
- 1rd5 - TrpS α subunit – corn
- 1ujp - TtTrpS α subunit
- 1v7y, 1wq5, 1xc4 - EcTrpS α subunit
- 1xcf - EcTrpS α subunit (mutant)
- 2ekc - TrpS α subunit – Aquifex aeolicus
- Tryptophan synthase α binary complex
- 1wxj - TtTrpS α subunit + indole propanol phosphate
- Tryptophan synthase β subunit
- 1x1q - TtTrpS β subunit – Thermus thermophilus
- 2dh6 - EcTrpS β subunit]] - Escherichia coli
- 2o2e, 2o2j - TrpS β subunit – Mycobacterium tuberculosis
- 1v8z - PfTrpS β subunit
- 4neg - TrpS β subunit – Bacillus anthracis
- 4qys - TrpS β subunit – Sulfolobus solfataricus
- Tryptophan synthase β binary complex
- 2dh5 - EcTrpS β subunit + PLP
- Tryptophan synthase α+β subunits
- 1wdw - PfTrpS α + β subunits
- Tryptophan synthase α+β binary complex
- 1ttp, 1ttq, 1bks, 2wsy, 1kfk - StTrpS α + β + PLP – Salmonella typhimurium
- 1a5a, 1k8x - StTrpS α (mutant) + β + PLP
- 1k7x, 2j9z - StTrpS α + β (mutant) + PLP
- 2tys - StTrpS α (mutant) + β (mutant) + Trp derivative
- 1kfe - StTrpS α (mutant) + β + pyridoxal-serine-monophsphate
- 1kfj - StTrpS α + β + pyridoxal-serine-monophsphate
- 2j9y - StTrpS α + β (mutant) + enoic acid derivative
- 3pr2 - StTrpS α + β + inhibitor
- 4hn4, 4hpj, 4hpx, 4ht3, 4kkx - TrpS α + β + inhibitor – Salmonella enterica
- Tryptophan synthase α+β ternary complex with PLP and inhibitor
- 1a50, 1cw2, 1c9d, 1cx9, 1c8v, 1c29, 2cle, 2clh, 2cli, 2clk, 2cll, 2clm, 2clo, 2clf - StTrpS α + β + inhibitor + PLP
- 1fuy - StTrpS α + β (mutant) + inhibitor + PLP
- Tryptophan synthase α+β ternary complex with PLP and IGP derivatives
- 1a5b, 1kfb - StTrpS α (mutant) + β + IGP + PLP
- 1k8z - StTrpS α + β (mutant) + IGP derivative + PLP
- 1kfc - StTrpS α (mutant) + β + IGP derivative + PLP
- 1k3u, 1k7e, 1k7f - StTrpS α + β + IGP derivative + PLP
- 1qoq, 2rh9, 2rhg - StTrpS α + β + IGP + PLP
- 1qop - StTrpS α + β + IGP analog + PLP
- 2trs, 1beu - StTrpS α (mutant) + β (mutant) + pyridoxal-serine-monophsphate + IGP analog
- Tryptophan synthase α+β ternary complex with PLP and G3P derivatives
- 1wbj - StTrpS α + β + G3P + PLP
- 2j9x - StTrpS α + β + G3P + acrylic acid derivative
- 1k8y - StTrpS α + β (mutant) + G3P derivative + PLP
- 1tjp - StTrpS α + β + G3P derivative + PLP
- 2tsy - StTrpS α (mutant) + β (mutant) + pyridoxal-serine-monophsphate + G3P
- 1ubs - StTrpS α (mutant) + β (mutant) + serine + PLP
- Tryptophan synthase α+β quaternary complex
- 1a5s - StTrpS α + β + serine + inhibitor + PLP
- 3cep - StTrpS α + β + G3P + indoline + PLP
References
