5b64 | pdb_00005b64

A novel binding mode of MAGUK GK domain revealed by DLG GK domain in complex with KIF13B MBS domain

spinqualitylabelsall models 5b64, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5b64 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GOL, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The membrane-associated guanylate kinase (MAGUK) scaffold proteins share a signature guanylate kinase (GK) domain. Despite their diverse functional roles in cell polarity control and synaptic signaling, the currently known mode of action of MAGUK GK is via its binding to phosphorylated short peptides from target proteins. Here, we discover that the GK domain of DLG MAGUK binds to an unphosphorylated and autonomously folded domain within the stalk region (MAGUK binding stalk [MBS] domain) of a kinesin motor KIF13B with high specificity and affinity. The structure of DLG4 GK in complex with KIF13B MBS reveals the molecular mechanism governing this atypical GK/target recognition mode and provides insights into DLG/KIF13B complex-mediated regulation of diverse cellular processes such as asymmetric cell division. We further show that binding to non-phosphorylated targets is another general property of MAGUK GKs, thus expanding the mechanisms of action of the MAGUK family proteins. An Atypical MAGUK GK Target Recognition Mode Revealed by the Interaction between DLG and KIF13B.,Zhu J, Shang Y, Xia Y, Zhang R, Zhang M Structure. 2016 Sep 15. pii: S0969-2126(16)30241-6. doi:, 10.1016/j.str.2016.08.008. PMID:27642159 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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