1pp5 | pdb_00001pp5

Structure of Antibacterial Peptide Microcin J25: a 21-Residue Lariat Protoknot

File:1pp5.gif

spinqualitylabelsall models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D load='1pp5' size='350' frame='false' scene=
1pp5
Gene:	MCJA (Escherichia coli)
Resources:	FirstGlance, OCA, PDBsum, RCSB
Coordinates:	save as pdb, mmCIF, xml

Overview

The antibacterial peptide microcin J25 (MccJ25) inhibits bacterial transcription by binding within, and obstructing, the nucleotide-uptake channel of bacterial RNA polymerase. Published covalent and three-dimensional structures indicate that MccJ25 is a 21-residue cycle. Here, we show that the published covalent and three-dimensional structures are incorrect, and that MccJ25 in fact is a 21-residue "lariat protoknot", consisting of an 8-residue cyclic segment followed by a 13-residue linear segment that loops back and threads through the cyclic segment. MccJ25 is the first example of a lariat protoknot involving a backbone-side chain amide linkage.

About this Structure

1PP5 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of antibacterial peptide microcin J25: a 21-residue lariat protoknot., Bayro MJ, Mukhopadhyay J, Swapna GV, Huang JY, Ma LC, Sineva E, Dawson PE, Montelione GT, Ebright RH, J Am Chem Soc. 2003 Oct 15;125(41):12382-3. PMID:14531661

Page seeded by OCA on Sun Mar 30 23:02:38 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA