3p7i | pdb_00003p7i

Crystal structure of Escherichia coli PhnD in complex with 2-aminoethyl phosphonate

spinqualitylabelsall models 3p7i, resolution 1.71Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3p7i is a 1 chain structure with sequence from Ecout. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GOL, P7I Gene: phnD, UTI89_C4699 (ECOUT) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The phnD gene of Escherichia coli encodes the periplasmic binding protein of the phosphonate (Pn) uptake and utilization pathway. We have crystallized and determined structures of E. coli PhnD (EcPhnD) in the absence of ligand and in complex with the environmentally abundant 2-aminoethylphosphonate (2AEP). Similar to other bacterial periplasmic binding proteins, 2AEP binds near the center of mass of EcPhnD in a cleft formed between two lobes. Comparison of the open, unliganded structure with the closed 2AEP-bound structure shows that the two lobes pivot around a hinge by approximately 70 degrees between the two states. Extensive hydrogen bonding and electrostatic interactions stabilize 2AEP, which binds to EcPhnD with low nanomolar affinity. These structures provide insight into Pn uptake by bacteria and facilitated the rational design of high signal-to-noise Pn biosensors based on both coupled small-molecule dyes and autocatalytic fluorescent proteins. Structure of the Escherichia coli Phosphonate Binding Protein PhnD and Rationally Optimized Phosphonate Biosensors.,Alicea I, Marvin JS, Miklos AE, Ellington AD, Looger LL, Schreiter ER J Mol Biol. 2011 Dec 2;414(3):356-69. Epub 2011 Oct 12. PMID:22019591 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also ABC transporter

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