4fb0 | pdb_00004fb0

Structure of Oceanobacillus iheyensis group II intron C377G mutant in a ligand-free state in the presence of K+ and Mg2+

spinqualitylabelsall models 4fb0, resolution 3.22Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4fb0 is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: EPE, K, MG, SPM Related: 4e8k, 4e8m, 4e8n, 4e8p, 4e8q, 4e8r, 4e8t, 4e8v, 4faq, 4far, 4fau, 4faw, 4fax Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Group II introns are self-splicing ribozymes that share a reaction mechanism and a common ancestor with the eukaryotic spliceosome, thereby providing a model system for understanding the chemistry of pre-mRNA splicing. Here we report 14 crystal structures of a group II intron at different stages of catalysis. We provide a detailed mechanism for the first step of splicing, we describe a reversible conformational change between the first and the second steps of splicing, and we present the ligand-free intron structure after splicing in an active state that corresponds to the retrotransposable form of the intron. During each reaction, the reactants are aligned and activated by a heteronuclear four-metal-ion center that contains a metal cluster and obligate monovalent cations, and they adopt a structural arrangement similar to that of protein endonucleases. Based on our data, we propose a model for the splicing cycle and show that it is applicable to the eukaryotic spliceosome. Visualizing Group II Intron Catalysis through the Stages of Splicing.,Marcia M, Pyle AM Cell. 2012 Oct 26;151(3):497-507. doi: 10.1016/j.cell.2012.09.033. PMID:23101623 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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