1rio | pdb_00001rio
Structure of bacteriophage lambda cI-NTD in complex with sigma-region4 of Thermus aquaticus bound to DNA
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| 1rio, resolution 2.30Å | |||||||||||||
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| Ligands: | CA, DA, DC, DG, DT, MPD, MSE | ||||||||||||
| Gene: | CI (Enterobacteria phage lambda) | ||||||||||||
| Related: | 1KU7, 1LMB
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Overview
The cI protein of bacteriophage lambda (lambdacI) activates transcription by binding a DNA operator just upstream of the promoter and interacting with the RNA polymerase sigma subunit domain 4 (sigma(4)). We determined the crystal structure of the lambdacI/sigma(4)/DNA ternary complex at 2.3 A resolution. There are no conformational changes in either protein, which interact through an extremely small interface involving at most 6 amino acid residues. The interactions of the two proteins stabilize the binding of each protein to the DNA. The results provide insight into how activators can operate through a simple cooperative binding mechanism but affect different steps of the transcription initiation process.
About this Structure
1RIO is a Protein complex structure of sequences from Enterobacteria phage lambda and Thermus aquaticus. Full crystallographic information is available from OCA.
Reference
Structure of a ternary transcription activation complex., Jain D, Nickels BE, Sun L, Hochschild A, Darst SA, Mol Cell. 2004 Jan 16;13(1):45-53. PMID:14731393
Page seeded by OCA on Sun Mar 30 23:28:40 2008
