5nbj | pdb_00005nbj
DLS Tetragonal - ReHEWL
Structural highlights
Function[LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.[1] Publication Abstract from PubMedMultiple possibilities for the coordination of fac-[Re(CO)3(H2O)3]+ to a protein have been determined and include binding to Asp, Glu, Arg and His amino-acid residues as well as to the C-terminal carboxylate in the vicinity of Leu and Pro. The large number of rhenium metal complex binding sites that have been identified on specific residues thereby allow increased target identification for the design of future radiopharmaceuticals. The core experimental concept involved the use of state-of-art tuneable synchrotron radiation at the Diamond Light Source to optimize the rhenium anomalous dispersion signal to a large value (f of 12.1 electrons) at its LI absorption edge with a selected X-ray wavelength of 0.9763 A. At the Cu Kalpha X-ray wavelength (1.5418 A) the f for rhenium is 5.9 electrons. The expected peak-height increase owing to the optimization of the Re f was therefore 2.1. This X-ray wavelength tuning methodology thereby showed the lower occupancy rhenium binding sites as well as the occupancies of the higher occupancy rhenium binding sites. New leads for fragment-based design of rhenium/technetium radiopharmaceutical agents.,Brink A, Helliwell JR IUCrJ. 2017 Apr 11;4(Pt 3):283-290. doi: 10.1107/S2052252517003475. eCollection, 2017 May 1. PMID:28512575[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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