Structural highlights
Function
[RAPZ_ECOLI] Modulates the synthesis of GlmS, by affecting the processing and stability of the regulatory small RNA GlmZ. When glucosamine-6-phosphate (GlcN6P) concentrations are high in the cell, RapZ binds GlmZ and targets it to cleavage by RNase E. Consequently, GlmZ is inactivated and unable to activate GlmS synthesis. Under low GlcN6P concentrations, RapZ is sequestered and inactivated by an other regulatory small RNA, GlmY, preventing GlmZ degradation and leading to synthesis of GlmS (PubMed:17824929, PubMed:23475961). Displays ATPase and GTPase activities in vitro. Can also hydrolyze pNPP (PubMed:19074378).[1] [2] [3]
References
- ↑ Kalamorz F, Reichenbach B, Marz W, Rak B, Gorke B. Feedback control of glucosamine-6-phosphate synthase GlmS expression depends on the small RNA GlmZ and involves the novel protein YhbJ in Escherichia coli. Mol Microbiol. 2007 Sep;65(6):1518-33. PMID:17824929 doi:https://dx.doi.org/10.1111/j.1365-2958.2007.05888.x
- ↑ Luciano J, Foulquier E, Fantino JR, Galinier A, Pompeo F. Characterization of YvcJ, a conserved P-loop-containing protein, and its implication in competence in Bacillus subtilis. J Bacteriol. 2009 Mar;191(5):1556-64. doi: 10.1128/JB.01493-08. Epub 2008 Dec 12. PMID:19074378 doi:https://dx.doi.org/10.1128/JB.01493-08
- ↑ Gopel Y, Papenfort K, Reichenbach B, Vogel J, Gorke B. Targeted decay of a regulatory small RNA by an adaptor protein for RNase E and counteraction by an anti-adaptor RNA. Genes Dev. 2013 Mar 1;27(5):552-64. doi: 10.1101/gad.210112.112. PMID:23475961 doi:https://dx.doi.org/10.1101/gad.210112.112
