5xex | pdb_00005xex

Crystal structure of S.aureus PNPase catalytic domain

spinqualitylabelsall models 5xex, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5xex is a 6 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GOL, PEG, PPV Activity: Polyribonucleotide nucleotidyltransferase, with EC number 2.7.7.8 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [PNP_STAA8] Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.[HAMAP-Rule:MF_01595] Publication Abstract from PubMed The RNA degradosome of the pathogen Staphylococcus aureus regulates the metabolism of RNA, the expression of virulence factors, and the formation of biofilms. It is composed of the RNases J1/J2, RNase Y, CshA, PNPase, Enolase, Pfk, and a newly identified component, RnpA. However, the function and new partners of RnpA in RNA degradosome remain unknown. Here, we identified PNPase and Enolase as two novel partners for RnpA. Further studies revealed that Enolase interacts with RnpA in competition with PNPase. Enzymatic assays showed that RnpA increases Enolase activity but has no effect on PNPase. These findings provide more information about the functional relationship between RnpA and RNA degradosome. Enolase binds to RnpA in competition with PNPase in Staphylococcus aureus.,Wang X, Wang C, Wu M, Tian T, Cheng T, Zhang X, Zang J FEBS Lett. 2017 Sep 27. doi: 10.1002/1873-3468.12859. PMID:28960276 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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