Crystal structure of Axin DIX domain
DIX dimer
About this Structure
1WSP is a 3 chains structure of sequences from Rattus norvegicus the DIX domain of Axin.
Publications
The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization.
Nat Struct Mol Biol. 2007 Jun;14(6):484-92. Epub 2007 May 27.
Schwarz-Romond T, Fiedler M, Shibata N, Butler PJ, Kikuchi A, Higuchi Y, Bienz M.
Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
Comment in: Nat Struct Mol Biol. 2007 Jun;14(6):463-5.
Publication Abstract from PubMed
The Wnt signaling pathway controls numerous cell fates in animal development and is also a major cancer pathway. Dishevelled (Dvl) transduces the Wnt signal by interacting with the cytoplasmic Axin complex. Dvl and Axin each contain a DIX domain whose molecular properties and structure are unknown. Here, we demonstrate that the DIX domain of Dvl2 mediates dynamic polymerization, which is essential for the signaling activity of Dvl2. The purified domain polymerizes gradually, reversibly and in a concentration dependent manner, ultimately forming fibrils. The Axin DIX domain has a novel structural fold largely composed of beta-strands that engage in head-to-tail self-interaction to form filaments in the crystal. The DIX domain thus seems to mediate the formation of a dynamic interaction platform with a high local concentration of binding sites for transient Wnt signaling partners; this represents a previously uncharacterized mechanistic principle, signaling by reversible polymerization.
The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization., Schwarz-Romond T, Fiedler M, Shibata N, Butler PJ, Kikuchi A, Higuchi Y, Bienz M, Nat Struct Mol Biol. 2007 Jun;14(6):484-92. Epub 2007 May 27. PMID:17529994
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
Full crystallographic information is available from OCA.
