EPSP synthase

spinqualitylabelsall models Structure of E. coli EPSP synthase complex with shikimate-3-phosphate, the herbicide glyphosate and formic acid (PDB entry 1g6s) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase is a key enzyme for the biosynthesis of aromatic amino acids in plants and many microbes. Consequently. EPSP synthase catalyzes the addition of phosphoenol pyruvate (PEP) to shikimate-3-phosphate (S3P), generating 5-enolpyruvylshikimate-3-phosphate, which is a precursor for phenylalanine and tyrosine. Relevance EPSP synthase is a target for drugs and herbicides like Roundup. Structural insights The enzyme has two domains, with the active site found in the interdomain cleft (open conformation). There is a substantial structural change upon substrate binding, resulting in a closed conformation. See animation of this process. Glyphosate (also known as Roundup) occupies the binding site of the second substrate, phosphoenol pyruvate .

3D structures of EPSP synthase

Updated on 19-October-2017

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• 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase
  • 1eps – EcEPSP – Escherichia coli
    
  • 1p88, 1p89 - EcEPSP N terminal - NMR
    
  • 1rf5 - SpEPSP – Streptococcus pneumoniae
    
  • 2bjb, 2o15 – MtEPSP – Mycobacterium tuberculosis
    
  • 2gg4 – AgEPSP – Agrobacterium
    
  • 3roi, 3tr1, 4gfp – CbEPSP – Coxiella burnetii
    
  • 3ti2 - VcEPSP N terminal – Vibrio cholerae
    
  • 3rmt – EPSP – Bacillus halodurans
    
• EPSP synthase binary complex
  • 1g6t - EcEPSP + S3P
    
  • 1mi4, 2qfq, 2qfs, 3fjx, 3fk0 - EcEPSP (mutant) + S3P
    
  • 1q36 - EcEPSP (mutant) + S3P derivative
    
  • 1x8r, 1x8t - EcEPSP + intermediate
    
  • 2aa9 – EcEPSP + shikimate
    
  • 2pq9 - EcEPSP + intermediate
    
  • 1rf4 - SpEPSP + intermediate
    
  • 2o0b, 2o0d - MtEPSP + S3P
    
  • 2gg6 - AgEPSP + S3P
    
  • 2pqb, 2pqc, 2pqd - AgEPSP + intermediate
    
  • 2o0x - MtEPSP + intermediate
    
  • 2o0z - MtEPSP + EPS
    
  • 4egr - CbEPSP + phosphoenolpyruvate
    
• EPSP synthase ternary complex
  • 1g6s - EcEPSP + S3P + glyphosate
    
  • 2qft, 2qfu, 3fjz, 3fk1 - EcEPSP (mutant) + S3P + glyphosate
    
  • 2aay - EcEPSP + shikimate + glyphosate
    
  • 1rf6 - SpEPSP + S3P + glyphosate
    
  • 2gga - AgEPSP + S3P + glyphosate
    
  • 2ggd - AgEPSP (mutant) + S3P + glyphosate
    
  • 2o0e - MtEPSP + S3P + phosphoenolpyruvate
    
  • 3nvs - VcEPSP + S3P + glyphosate
    
  • 3slh - CbEPSP + S3P + glyphosate
    

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