3fto | pdb_00003fto

Crystal structure of OppA in a open conformation

spinqualitylabelsall models 3fto, resolution 2.38Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3fto is a 1 chain structure with sequence from "streptococcus_hollandicus"_scholl_1891 "streptococcus hollandicus" scholl 1891. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. NonStd Res: MSE Related: 3drf, 3drg, 3drh, 3dri, 3drj, 3drk Gene: llmg_0701, oppA ("Streptococcus hollandicus" Scholl 1891) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Lactococcus lactis is a promising host for (membrane) protein overproduction. Here, we describe a protocol for incorporation of selenomethionine (SeMet) into proteins expressed in L. lactis. Incorporation efficiencies of SeMet in the membrane protein complex OpuA (an ABC transporter) and the soluble protein OppA, both from L. lactis, were monitored by mass spectrometry. Both proteins incorporated SeMet with high efficiencies (>90%), which greatly extends the usefulness of the expression host L. lactis for X-ray crystallography purposes. The crystal structure of ligand-free OppA was determined at 2.4 A resolution by a semiautomatic approach using selenium single-wavelength anomalous diffraction phasing. Selenomethionine incorporation in proteins expressed in Lactococcus lactis.,Berntsson RP, Alia Oktaviani N, Fusetti F, Thunnissen AM, Poolman B, Slotboom DJ Protein Sci. 2009 May;18(5):1121-7. PMID:19388077 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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