3wfr | pdb_00003wfr

tRNA processing enzyme complex 2

spinqualitylabelsall models 3wfr, resolution 3.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3wfr is a 8 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CTP, MG, POP NonStd Res: UNK Related: 3wfo, 3wfp, 3wfq, 3wfs Activity: CCA tRNA nucleotidyltransferase, with EC number 2.7.7.72 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The 3'-terminal CCA (CCA-3' at positions 74-76) of tRNA is synthesized by CCA-adding enzyme using CTP and ATP as substrates, without a nucleic acid template. In Aquifex aeolicus, CC-adding and A-adding enzymes collaboratively synthesize the CCA-3'. The mechanism of CCA-3' synthesis by these two enzymes remained obscure. We now present crystal structures representing CC addition onto tRNA by A. aeolicus CC-adding enzyme. After C(7)(4) addition in an enclosed active pocket and pyrophosphate release, the tRNA translocates and rotates relative to the enzyme, and C(7)(5) addition occurs in the same active pocket as C(7)(4) addition. At both the C(7)(4)-adding and C(7)(5)-adding stages, CTP is selected by Watson-Crick-like hydrogen bonds between the cytosine of CTP and conserved Asp and Arg residues in the pocket. After C(7)(4)C(7)(5) addition and pyrophosphate release, the tRNA translocates further and drops off the enzyme, and the CC-adding enzyme terminates RNA polymerization. Translocation and rotation of tRNA during template-independent RNA polymerization by tRNA nucleotidyltransferase.,Yamashita S, Takeshita D, Tomita K Structure. 2014 Feb 4;22(2):315-25. doi: 10.1016/j.str.2013.12.002. Epub 2014 Jan, 2. PMID:24389024 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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