2a6q | pdb_00002a6q
| |||||||||||||
| 2a6q, resolution 2.05Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene: | yefM (Escherichia coli), yoeB (Escherichia coli) | ||||||||||||
| Related: | 2A6R, 2A6S
| ||||||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of YefM-YoeB complex
Overview
The eubacterial chromosome encodes various addiction modules that control global levels of translation through RNA degradation. Crystal structures of the Escherichia coli YefM2 (antitoxin)-YoeB (toxin) complex and the free YoeB toxin have been determined. The structure of the heterotrimeric complex reveals an asymmetric disorder-to-order recognition strategy, in which one C terminus of the YefM homodimer exclusively interacts with an atypical microbial ribonuclease (RNase) fold of YoeB. Comparison with the YefM-free YoeB structure indicates a conformational rearrangement of the RNase catalytic site of YoeB, induced by interaction with YefM. Complementary biochemical experiments demonstrate that the YoeB toxin has an in vitro RNase activity that preferentially cleaves at the 3' end of purine ribonucleotides.
About this Structure
2A6Q is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Conformational change in the catalytic site of the ribonuclease YoeB toxin by YefM antitoxin., Kamada K, Hanaoka F, Mol Cell. 2005 Aug 19;19(4):497-509. PMID:16109374
Page seeded by OCA on Mon Mar 31 01:48:39 2008
