4k8z | pdb_00004k8z

KOD Polymerase in binary complex with dsDNA

spinqualitylabelsall models 4k8z, resolution 2.29Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4k8z is a 3 chain structure with sequence from Pyrococcus kodakaraensis (strain kod1). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: EDO, MG, NCO NonStd Res: 2DA Related: 1wns, 1qht, 4k8x Gene: pol, pol TK0001, TK0001 (Pyrococcus kodakaraensis (strain KOD1)) Activity: DNA-directed DNA polymerase, with EC number 2.7.7.7 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [DPOL_PYRKO] Intein encoded endonucleases are thought to mediate intein mobility by site-specific recombination initiated by endonuclease cleavage at the "homing site" in gene that lack the intein. PI-PkoI recognizes 5'-GATTTAGATCCCTGTACC-3' and PI-PkoII recognizes 5'-CAGCTACTACGGTTAC-3'. Publication Abstract from PubMed Replicate it: Structures of KOD and 9 degrees N DNA polymerases, two enzymes that are widely used to replicate DNA with highly modified nucleotides, were solved at high resolution in complex with primer/template duplex. The data elucidate substrate interaction of the two enzymes and pave the way for further optimisation of the enzymes and substrates. Structures of KOD and 9 degrees N DNA Polymerases Complexed with Primer Template Duplex.,Bergen K, Betz K, Welte W, Diederichs K, Marx A Chembiochem. 2013 Jun 17;14(9):1058-62. doi: 10.1002/cbic.201300175. Epub 2013, Jun 3. PMID:23733496 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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