3mm0 | pdb_00003mm0

Crystal structure of chimeric avidin

spinqualitylabelsall models 3mm0, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3mm0 is a 12 chain structure with sequence from Chick. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 2avi, 1y53 Gene: AVR4, AVR5, AVD (CHICK) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [AVID_CHICK] The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin). Publication Abstract from PubMed Avidin and its bacterial analogue streptavidin have been widely used in applications in life sciences. Recently, we described a highly thermostable engineered avidin, called chimeric avidin, which is a hybrid of avidin and avidin related protein 4. Here we report a protocol for pilot-scale production in E. coli and the X-ray structure of the Ile117Tyr mutant of chimeric avidin. The ligand-binding properties of chimeric avidin were explored with isothermal titration calorimetry. We found chimeric avidin to be more stable against various harsh organic solvents at elevated temperatures compared to avidin and streptavidin. The properties of chimeric avidin make it a potential tool for new applications in biotechnology. (c) 2010 Wiley Periodicals, Inc. Chimeric avidin shows stability against harsh chemical conditions - biochemical analysis and 3D structure.,Maatta JA, Eisenberg-Domovich Y, Nordlund HR, Hayouka R, Kulomaa MS, Livnah O, Hytonen VP Biotechnol Bioeng. 2010 Oct 11. PMID:20939005 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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