6elk | pdb_00006elk

C.elegans MnSOD-3 mutant - Q142H

spinqualitylabelsall models 6elk, resolution 1.65Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6elk is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GOL, MN, SO4 Related: 3dc5, 4x9q Activity: Superoxide dismutase, with EC number 1.15.1.1 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [SODM2_CAEEL] Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. Publication Abstract from PubMed We have generated a site-directed mutant of the manganese superoxide dismutase SOD-3 of C.elegans (MnSOD-3) which modifies the metal specificity of the enzyme. While wild-type MnSOD-3 functions with manganese in the active site (3600 U.mg protein-1) it has little or no activity when iron is incorporated. However, when histidine replaces glutamine 142 in the active site, the enzyme retains 50% of its activity and becomes cambialistic for its metal cofactor exhibiting very similar specific activity with either manganese or iron. A single mutation is sufficient to modify the metal selectivity and specificity of a eukaryotic manganese superoxide dismutase to encompass iron.,Hunter GJ, Hunter T, Trinh C, Bonetta R, Sacco A, Vella M, Sultana PM, Borowski T, Garcia-Fandino R, Stockner T, Fadia H Chemistry. 2017 Nov 26. doi: 10.1002/chem.201704655. PMID:29178484 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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