Structural highlights
Function[LYS_BPCP7] Responsible for the separation of the host daughter cells at the end of cell division and participates in the liberation of progeny bacteriophage into the medium. Degrades cell walls containing either choline or ethanolamine. Publication Abstract from PubMedEndolysins, the cell wall lytic enzymes encoded by bacteriophages to release the phage progeny, are among the top alternatives to fight against multiresistant pathogenic bacteria; one of the current biggest challenges to global health. Their narrow range of susceptible bacteria relies, primarily, on targeting specific cell-wall receptors through specialized modules. The cell wall-binding domain of Cpl-7 endolysin, made of three CW_7 repeats, accounts for its extended-range of substrates. Using as model system the cell wall-binding domain of Cpl-7, here we describe the molecular basis for the bacterial cell wall recognition by the CW_7 motif, which is widely represented in sequences of cell wall hydrolases. We report the crystal and solution structure of the full-length domain, identify N-acetyl-D-glucosaminyl-(beta1,4)-N-acetylmuramyl-L-alanyl-D-isoglutamine (GMDP) as the peptidoglycan (PG) target recognized by the CW_7 motifs, and characterize feasible GMDP-CW_7 contacts. Our data suggest that Cpl-7 cell wall-binding domain might simultaneously bind to three PG chains, and also highlight the potential use of CW_7-containing lysins as novel anti-infectives. Deciphering how Cpl-7 cell wall-binding repeats recognize the bacterial peptidoglycan.,Bustamante N, Iglesias-Bexiga M, Bernardo-Garcia N, Silva-Martin N, Garcia G, Campanero-Rhodes MA, Garcia E, Uson I, Buey RM, Garcia P, Hermoso JA, Bruix M, Menendez M Sci Rep. 2017 Nov 28;7(1):16494. doi: 10.1038/s41598-017-16392-4. PMID:29184076[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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