5wuj | pdb_00005wuj
Crystal structure of FliF-FliG complex from H. pylori
Structural highlights
Function[O25118_HELPY] The M ring may be actively involved in energy transduction.[PIRNR:PIRNR004862] [FLIG_HELPY] One of the proteins that forms a switch complex that is proposed to be located at the base of the basal body. This complex interacts with chemotaxis proteins (such as CheY) in addition to contacting components of the motor that determine the direction of flagellar rotation. Required for flagellum synthesis and motility. In H.pylori four flagellar switch proteins are encoded, FliG, FliM, FliN and FliY.[1] [2] Publication Abstract from PubMedThe bacterial flagellar motor is a self-assembling supramolecular nanodevice. Its spontaneous biosynthesis is initiated by the insertion of the MS ring protein FliF into the inner membrane, followed by attachment of the switch protein FliG. Assembly of this multiprotein complex is tightly regulated to avoid nonspecific aggregation, but the molecular mechanisms governing flagellar assembly are unclear. Here, we present the crystal structure of the cytoplasmic domain of FliF complexed with the N-terminal domain of FliG (FliFC-FliGN) from the bacterium Helicobacter pylori Within this complex, FliFC interacted with FliGN through extensive hydrophobic contacts similar to those observed in the FliFC-FliGN structure from the thermophile Thermotoga maritima, indicating conservation of the FliFC-FliGN interaction across bacterial species. Analysis of the crystal lattice revealed that the heterodimeric complex packs as a linear superhelix via stacking of the armadillo-repeat-like motifs (ARM) of FliGN Notably, this linear helix was similar to that observed for the assembly of the FliG middle domain. We validated the in vivo relevance of the FliGN stacking by complementation studies in Escherichia coli. Furthermore, structural comparison with apo FliG from the thermophile Aquifex aeolicus indicated that FliF regulates the conformational transition of FliG and exposes the complementary ARM-like motifs of FliGN, containing conserved hydrophobic residues. FliF apparently both provides a template for FliG polymerization and spatiotemporally controls subunit interactions within FliG. Our findings reveal that a small protein fold can serve as a versatile building block to assemble into a multiprotein machinery of distinct shapes for specific functions. Crystal structure of the FliF-FliG complex from Helicobacter pylori yields insight into the assembly of the motor MS-C ring in the bacterial flagellum.,Xue C, Lam KH, Zhang H, Sun K, Lee SH, Chen X, Au SWN J Biol Chem. 2017 Dec 11. pii: M117.797936. doi: 10.1074/jbc.M117.797936. PMID:29229777[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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